Mouse recombinant monoclonal antibodies are produced by introducing the genes encoding the antibody into mammalian cells using recombinant DNA technology. These versatile antibodies find widespread applications in research, serving as essential tools in laboratories for techniques such as Western blotting, immunoprecipitation, immunofluorescence, and flow cytometry. Their pivotal role in studying protein expression, localization, and function underscores their significance in advancing scientific knowledge.
Despite challenges related to immunogenicity in humans, innovative strategies, including humanization and the creation of chimeric antibodies, aim to mitigate these concerns. In diagnostics, mouse recombinant monoclonal antibodies are pivotal in detecting specific proteins or markers associated with diseases. Immunoassays like enzyme-linked immunosorbent assays (ELISA) and lateral flow assays benefit from the precision and reliability these antibodies bring to diagnostic applications.