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The HuProt™ microarray by CDI Labs contains the world’s largest and most comprehensive
collection of thoroughly validated human proteins.
HuProt™ consists of over 21,000 unique human proteins, isoform variants and protein fragments – covering 16,794 unique genes, it is the most comprehensive collection of human proteins available. Included are 15,889 of the 19,613 canonical human proteins described in Human Protein Atlas, with broad coverage across protein subclasses.
Protein libraries and analysis services for antibody seromics, biomarker discovery, cross-reactivity screening, and other whole-proteome target identification and interaction assay.
CDI Labs’ non-contact piezoelectric ‘inkjet’ process uses next-generation Arrayjet printers and allows for rapid production of high-quality microarray slides time after time. In comparison to older contact pin printing methods – HuProt microarrays are made with improved accuracy and reproducibility with excellent spot morphology.
Pooled human plasma was incubated on pairs of HuProt proteome microarrays across two print batches and stained with a fluorescent anti-IgG secondary antibody. Raw median fluorescence intensity data were captured, log2 transformed, and plotted. Linear regression analysis demonstrates reproducibility across the array pair from Lot 1 (R2 = 0.96), Lot 2 ( R2 = 0.92), and the averaged data from both array batches (R2 = 0.96). These results demonstrate the robust reproducibility of HuProt autoantibody seromics data both within and across microarray production lots.
Protein purification prior to microarray manufacturing selects for proteins with correctly folded GST-fusion proteins. Testing on the HuProt microarray demonstrates that the proteins are folded in native conformation and retain function (Hu S. poster; Venkataraman A., et al., 2018). When both native and denatured HuProt microarrays were probed with monoclonal antibodies that selectively recognize either linear or folded epitopes of their cognate antigen, the antibodies were found to recognize the appropriate antigen form (Venkataraman A., et al., 2018). Further tests (RNA binding) demonstrated that proteins on HuProt do retain function (Venkataraman A., et al., 2018).
Production technology: Non-contact piezoelectric inkjet
Slide type: Coated glass (PATH)
Slide dimensions: 75 x 25 x 1 mm
Detection method: Fluorescence
Content: Recombinant human and mouse proteins
CDI Labs offers transformational tools and services that empower researchers to unravel the connection between autoantibodies and disease. CDI offers the HuProt™ Human Proteome Microarray – the most comprehensive collection of correctly-folded, full-length human proteins, and VirScan®, HuScan®, and MouseScan™ Phage ImmunoPrecipitation Sequencing (PhIP-Seq) services. Application areas include autoantibody seromics, target identification and antibody specificity validation.
