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14-3-3 Sigma / Stratifin Antibody [CPTC-SFN-2]

In Stock
Catalog Number Formulation Size Price
2810-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2810-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2810-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Nucleus, Secreted
Gene Name
SFN
Positive Control
A-431 cell lines., hTCEpi, PC-3 cells, Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT
Immunogen
Recombinant human full-length SFN protein
Alternate Names
14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN; HME1; Stratifin; YWHAS; 14-3-3 Sigma

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
CPTC-SFN-2
Chromosome Location
1p36.11
Mol. Weight of Antigen
28kDa

Functions

  • Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
  • p53-regulated inhibitor of G2/M progression.

Key References

  • Pulukuri, S M, and J S Rao. Oncogene., U.S. National Library of Medicine, 3 Aug. 2006.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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