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Recombinant 14-3-3E / Tryptophan 5-Monooxygenase Antibody [YWHAE/8636R]

In Stock
Catalog Number Formulation Size Price
7531-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7531-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7531-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant 14-3-3E / Tryptophan 5-Monooxygenase Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with 14-3-3E Recombinant Rabbit Monoclonal Antibody (YWHAE/8636R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant 14-3-3E / Tryptophan 5-Monooxygenase Antibody - Image 2

SDS-PAGE Analysis of Purified 14-3-3E Recombinant Rabbit Monoclonal Antibody (YWHAE/8636R). Confirmation of Purity and Integrity of Antibody.

YWHAE Antibody in Western Blot (WB)

YWHAE Antibody in Western Blot (WB)

Western blot analysis of 293T, PC-3 and A431 lysates using YWHAE Recombinant Rabbit Monoclonal Antibody (YWHAE/8636R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3E has also been identified as a possible susceptibility gene for schizophrenia.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, AKT Signaling, Defective Intrinsic Apoptosis, Infectious Disease, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
YWHAE
Positive Control
HEK-293 or A-431 cell lines. PC-3, Human brain|prostate or placenta.
Immunogen
Recombinant human full-length YWHAE protein
Alternate Names
14-3-3 protein epsilon, 14-3-3E, MDS; epididymis luminal protein 2; mitochondrial import stimulation factor (MSF) L subunit; Mitochondrial import stimulation factor L subunit; MSF L; protein kinase C inhibitor protein-1

Database Links

Entrez Gene ID
7531
SwissProt
P62258

Additional Information

Clone
YWHAE/8636R
Chromosome Location
17p13.3
Mol. Weight of Antigen
29.4kDa

Related Antibodies

View All YWHAE Antibodies

Functions

  • Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Plays an important role in translational accuracy. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

Key References

  • Ikeda, Masashi, and Takao Hikita. OUP Academic, Oxford University Press, 24 July 2008.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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