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A20 / TNFAIP3 (Marker of Tamoxifen Resistance) Antibody [TNFAIP3/2813]

In Stock
Catalog Number Formulation Size Price
7128-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7128-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7128-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).

A20 / TNFAIP3 Antibody in SDS-PAGE

A20 / TNFAIP3 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified A20 / TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

A20 (also known as TNFAIP3) is a zinc finger protein and inhibits NF-kappa B activation as well as TNF-mediated apoptosis. The protein has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. A20 is as a tumor suppressor and has been shown to protect MCF-7 breast carcinoma cells from TNF-induced apoptosis. HighA20expression levels were observed in more aggressive breast tumors (ER-negative, progesterone receptor-negative and high histological grade). These findings strongly suggest that A20 is a key protein involved in tamoxifen resistance.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, Immunology, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Cytoplasm, Lysosome, Nucleus
Gene Name
TNFAIP3
Positive Control
Hodgkin s Lymphoma or hepatocellular carcinoma (HCC).
Immunogen
Recombinant fragment (around aa 241-356) of human TNFAIP3 protein (exact sequence is proprietary)
Alternate Names
Tumor necrosis factor alpha-induced protein 3, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20, A20; Putative DNA-binding protein A20; TNF alpha-induced protein 3 (TNFAIP3); Tumor Necrosis Factor alpha-Induced Protein 3(TNFAIP3); Zinc finger protein A20

Database Links

Entrez Gene ID
7128
SwissProt
P21580

Additional Information

Clone
TNFAIP3/2813
Chromosome Location
6q23
Mol. Weight of Antigen
~90kDa

Related Antibodies

View All TNFAIP3 Antibodies

Functions

  • Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of pro-inflammatory cytokines and IFN beta in LPS-tolerized macrophages.

Key References

  • Schmitz R, et al. TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma. J Exp Med. 2009 May 11; 206(5):981-9.
  • J A Vendrell,et al. A20/TNFAIP3, a new estrogen-regulated gene that confers tamoxifen resistance in breast cancer cells. Oncogene,26:4656-4667; 2007.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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