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Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody [1A4 + ACTA2/791]

In Stock
Catalog Number Formulation Size Price
59-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
59-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
59-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Formalin-fixed, paraffin-embedded Rat Stomach stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Formalin-fixed, paraffin-embedded Rat Stomach stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Smooth Muscle Actin MAb (1A4 + ACTA2/791).

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Smooth Muscle Actin MAb (1A4 + ACTA2/791).

Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with Smooth Muscle Actin MAb (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).

Immunofluorescence Analysis of HeLa cells labeling Smooth Muscle Actin with Smooth Muscle Actin MAb (1A4 + ACTA2/791) followed by Goat anti-Mouse IgG-CF488(Green). The nuclear counterstain is NucSpot (Red).

SDS-PAGE Analysis Purified Smooth Muscle Actin MAb (1A4 + ACTA2/791). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis Purified Smooth Muscle Actin MAb (1A4 + ACTA2/791). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Rat
Research Areas
Cardiovascular, Mesenchymal Stem Cell Differentiation, Signal Transduction
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm, Cytoskeleton
Gene Name
ACTA2
Positive Control
Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
Immunogen
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH (1A4); Recombinant full-length human ACTA2 protein (ACTA2/791)
Alternate Names
Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Database Links

Entrez Gene ID
59
SwissProt
P62736

Additional Information

Clone
1A4 + ACTA2/791
Chromosome Location
10q23.31
Mol. Weight of Antigen
42kDa

Related Antibodies

View All ACTA2 Antibodies

Functions

  • Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Key References

  • Gown, A.M., et al. 1985. A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes. J. Cell Biol. 100: 807-813.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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