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AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [SPM334]

In Stock
Catalog Number Formulation Size Price
174-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
174-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
174-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody

Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Monoclonal Antibody (SPM334).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Dog, Human, Monkey, Pig
Research Areas
Cardiovascular, Stem Cell Differentiation
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Secreted
Gene Name
AFP
Positive Control
Hep-G2 cells. Fetal liver or hepatocellular carcinoma.
Immunogen
Alpha feto protein (AFP) Purified from serum of a hepatoma patient
Alternate Names
Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha fetoglobulin; FETA; HPAFP

Database Links

Entrez Gene ID
174
SwissProt
P02771

Additional Information

Clone
SPM334
Chromosome Location
4q13.3
Mol. Weight of Antigen
70kDa

Related Antibodies

View All AFP Antibodies

Functions

  • Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Key References

  • Yazova AK; Goussev AI; Poltoranina VS; Yakimenko EF. Human alpha-fetoprotein epitopes as revealed by monoclonal antibodies. Immunology Letters, 1990 Sep, 25(4):325-30.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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