AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [C2 + C3 + MBS-12]

Name: AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody
SKU: 174-MSM2
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Formalin-fixed, paraffin-embedded human Fetal Liver stained with AFP Mouse Monoclonal Antibody (C2 + C3 + MBS-12).

Immunofluorescence Analysis of PFA-fixed HepG2 cells labeling AFP using AFP Mouse Monoclonal Antibody (C2 + C3 + MBS-12) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.

Applications & Dilutions

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Immunofluorescence, Immunohistochemistry

Species Reactivity

Human

Research Areas

Cardiovascular, Stem Cell Differentiation

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Secreted

Gene Name

AFP

Positive Control

HepG2 cells. Fetal liver or hepatocellular carcinoma.

Immunogen

Recombinant full-length human Alpha feto protein

Alternate Names

Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha fetoglobulin; FETA; HPAFP

Database Links

Entrez Gene ID

174

SwissProt

P02771

Additional Information

Clone

C2 + C3 + MBS-12

Chromosome Location

4q13.3

Mol. Weight of Antigen

70kDa

Functions

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Key References

Lafuste, P., et al. 2002. -fetoprotein gene expression in early and full- term human trophoblast. Placenta 23: 600-612.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [C2 + C3 + MBS-12]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

Related Antibodies

Recombinant AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [AFP/7007R]

Recombinant AFP (Alpha Fetoprotein) (Hepatocellular / Germ Cell Tumor Marker) Antibody [rAFP/7257]

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [C3]

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [SPM334]

AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker) Antibody [MBS-12]