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Recombinant Aldo-keto Reductase Family 1 Member B1 (Adrenal Marker) Antibody [AKR1B1/7009R]

In Stock
Catalog Number Formulation Size Price
231-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
231-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
231-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml.
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human kidney stained with Aldose Reductase Recombinant Rabbit Monoclonal Antibody (AKR1B1/7009R).

Formalin-fixed, paraffin-embedded human kidney stained with Aldose Reductase Recombinant Rabbit Monoclonal Antibody (AKR1B1/7009R).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Staining using AKR1B1/7009R at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Staining using AKR1B1/7009R at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified AKR1B1 Rabbit Monoclonal Antibody (AKR1B1/7009R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified AKR1B1 Rabbit Monoclonal Antibody (AKR1B1/7009R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

AKR1B1, also designated as aldose reductase, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. It has also been shown to have decreased expression in adrenocortical cancer, and possibly play a role in adrenal tumorigenesis. It has been suggested that AKR1B1 could be investigated as a marker of malignancy for adrenal tumor diagnosis.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm
Gene Name
AKR1B1
Positive Control
Jurkat or Raji cells. Human kidney or colon carcinoma., MOLT4
Immunogen
Recombinant human full-length AKR1B1 protein
Alternate Names
Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AKR1B1; Aldehyde reductase; aldehyde reductase 1; Aldo-keto reductase family 1 member B1; aldo-keto reductase family 1, member B1 (aldose reductase); Aldose reductase; AR; Lii5-2 CTCL tumor antigen; low Km aldose reductase

Database Links

Entrez Gene ID
231
SwissProt
P15121

Additional Information

Clone
AKR1B1/7009R
Chromosome Location
7q33
Mol. Weight of Antigen
37kDa

Related Antibodies

View All AKR1B1 Antibodies

Functions

  • Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:21329684).

Key References

  • Bohren, K.M., et al. J. Biol. Chem. 264: 9547-9551.
  • Lefranc ois-Martinez, Anne-Marie, et al. OUP Academic, Oxford University Press, 1 June 2004.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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