Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker) Antibody [AACT/1451 + AACT/1452]

Name: Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker) Antibody
SKU: 12-MSM13
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Alpha-1-Antichymotrypsin (SERPINA3) (Histiocytoma Marker) Antibody

Formalin-fixed, paraffin-embedded human pancreas stained with Alpha-1-Antichymotrypsin Monoclonal Antibody (AACT/1451 + AACT/1452).

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a protein of 65-76kDa, which is identified antichymotrypsin (AACT). AACT is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of 伪 1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants; their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACTantibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Immunohistochemistry

Species Reactivity

Human

Research Areas

Cardiovascular, Immunology

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Secreted

Gene Name

SERPINA3

Positive Control

HeLa cells. Human tonsil, pancreas or histiocytoma.

Immunogen

Recombinant human Antichymotrypsin (AACT) protein fragment (around aa 49-187) (exact sequence is proprietary)

Alternate Names

Alpha-1-antichymotrypsin, Cell growth-inhibiting gene 24/25 protein, Serpin A3, SERPINA3; AACT; ACT; Alpha-1-antichymotrypsin; Antichymotrypsin; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; Growth inhibiting protein 24; Growth inhibiting protein 25; Serine (or cysteine) proteinase inhibitor clade A member 3; Serine proteinase inhibitor clade A member 3; Serpin A3; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3

Database Links

Entrez Gene ID

SwissProt

P01011

Additional Information

Clone

AACT/1451 + AACT/1452

Chromosome Location

14q32.1

Mol. Weight of Antigen

65-76kDa

Functions

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

Key References

Miyake, H., et al. 2001. Value of prostate specific antigen α1-antichymotrypsin complex for the detection of prostate cancer in patients with a PSA level of 4.1-10.0 ng/mL: comparison with PSA-related parameters. Int. J. Urol. 8: 589-593.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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