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AMACR / p504S (Prostate Cancer Marker) Antibody [AMACR/4757]

In Stock
Catalog Number Formulation Size Price
23600-MSM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
23600-MSM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
23600-MSM17-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
AMACR / p504S (Prostate Cancer Marker) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AMACR Mouse Monoclonal Antibody (AMACR/4757). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

AMACR / p504S (Prostate Cancer Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human colon carcinoma stained with AMACR Mouse Monoclonal Antibody (AMACR/4757). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

AMACR / p504S (Prostate Cancer Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified AMACR Mouse Monoclonal Antibody (AMACR/4757). Confirmation of Purity and Integrity of Antibody.

AMACR Antibody in Western Blot (WB)

AMACR Antibody in Western Blot (WB)

Western Blot Analysis of human kidney tissue lysate using AMACR Mouse Monoclonal Antibody (AMACR/4757).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody recognizes a protein of 42kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and β-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm.
Gene Name
AMACR
Positive Control
liver or prostate adenocarcinoma.|PC3 or HEK cells. Human kidney
Immunogen
Recombinant full-length human AMACR protein
Alternate Names
Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

Database Links

Entrez Gene ID
23600
SwissProt
Q9UHK6

Additional Information

Clone
AMACR/4757
Chromosome Location
5p13.3
Mol. Weight of Antigen
42kDa

Related Antibodies

View All AMACR Antibodies

Functions

  • Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).

Key References

  • Jiang Z et. al. Human Pathology. 2003; 34(8):792
  • Jiang Z et. al. Am J Surg Pathol. 2001; 25(11):1397

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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