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Recombinant Annexin A1 / (Hairy Cell Leukemia Marker) Antibody [rANXA1/9145]

In Stock
Catalog Number Formulation Size Price
301-MSM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
301-MSM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
301-MSM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Annexin A1 Antibody in SDS-PAGE

Annexin A1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified ANXA1 Recombinant Mouse Monoclonal Antibody (rANXA1/9145). Confirmation of Purity and Integrity of Antibody.

Annexin A1 Antibody in Immunohistochemistry (IHC (P))

Annexin A1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with Annexin A1 Recombinant Mouse Monoclonal Antibody (rANXA1/9145). Inset: PBS instead of primary antibody; secondary only negative control.

Annexin A1 Antibody in Western Blot (WB)

Annexin A1 Antibody in Western Blot (WB)

Western Blot Analysis of A549 lysate using Annexin A1 Mouse Recombinant Monoclonal Antibody (rANXA1/9145).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Cytokine Signaling, Dendritic Cell Marker, Signal Transduction
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium, Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium
Gene Name
ANXA1
Positive Control
HeLa or A549 cells. Human esophagus, placenta or prostate.
Immunogen
Recombinant full-length human Annexin A1 protein
Alternate Names
Annexin A1 | Annexin Ac2-26, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35, ANXA1; ANX1; Annexin-1; Chromobindin-9; LPC1; p35

Database Links

Entrez Gene ID
301
SwissProt
P04083

Additional Information

Clone
rANXA1/9145
Chromosome Location
9q21.13
Mol. Weight of Antigen
38kDa

Related Antibodies

View All ANXA1 Antibodies

Functions

  • Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).
  • Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:22879591, PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591).

Key References

  • Falini B., et al. Lancet 363: 1869-1871, Jun 5, 2004.
  • Basso K., et al.J Exp Med199:59-68, 2004.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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