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Arginase 1 (Hepatocellular Carcinoma Marker) Antibody [ARG1/9447]

In Stock
Catalog Number Formulation Size Price
383-MSM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
383-MSM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
383-MSM17-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Arginase 1 Antibody in Immunohistochemistry (IHC (P))

Arginase 1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma (HCC) stained with Arginase 1 Mouse Monoclonal Antibody (ARG1/9447). Inset: PBS instead of primary antibody; secondary only negative control.

Arginase 1 Antibody in Western Blot (WB)

Arginase 1 Antibody in Western Blot (WB)

Western Blot Analysis of human liver tissue lysate using Arginase 1 Mouse Monoclonal Antibody (ARG1/9447).

Arginase 1 Antibody in SDS-PAGE

Arginase 1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Arginase 1 Mouse Monoclonal Antibody (ARG1/9447). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Dendritic Cell Marker
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm, Cytoplasmic granule
Gene Name
ARG1
Positive Control
Human Liver
Immunogen
Recombinant fragment (around aa1-200) of human ARG1 protein(exact sequence is proprietary)
Alternate Names
Arginase-1, Liver-type arginase, Type I arginase, Arginase 1; ARG1; liver-type arginase; type I arginase

Database Links

Entrez Gene ID
383
SwissProt
P05089

Additional Information

Clone
ARG1/9447
Chromosome Location
6q23
Mol. Weight of Antigen
35-38kDa

Related Antibodies

View All ARG1 Antibodies

Functions

  • Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.
  • Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion (PubMed:15546957, PubMed:16709924, PubMed:19380772). In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Key References

  • Diez, A., et al. 1994. Immunological identity of the two different molecular mass constitutive subunits of liver arginase. Biol. Chem. Hoppe Seyler 375: 537-541.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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