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ARID1A / SMARCF1 Antibody [ARID1A/7735]

In Stock
Catalog Number Formulation Size Price
8289-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
8289-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
8289-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
ARID1A / SMARCF1 Antibody

Formalin-fixed, paraffin-embedded human ovarian cancer stained with ARID1A Mouse Monoclonal Antibody (ARID1A/7735). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2a) and Brg-1 (also designated SNF2b) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits, such as BAF250a (p270 or ARID1A) and BAF250b (ARID1B), are thought to play regulatory roles.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Nucleus.
Gene Name
ARID1A
Positive Control
and PBL.|colon|Highly expressed in spleen|ovary|prostate|Small Intestine|testis|thymus
Immunogen
Recombinant fragment (around aa1900-2000) of human ARID1A protein (exact sequence is proprietary)
Alternate Names
AT-rich interactive domain-containing protein 1A, B120, BRG1-associated factor 250, BRG1-associated factor 250a, Osa homolog 1, SWI-like protein, SWI/SNF complex protein p270, SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1, hELD, actin-dependent regulator of chromatin subfamily F member 1; ARID domain containing protein 1A; AT rich interactive domain 1A (SWI like); AT rich interactive domain containing protein 1A; B120; BAF250; BAF250A; BM029; brain protein 120; BRG1-associated factor 250a; C1ORF4; chromatin remodeling factor p250; chromosome 1 open reading frame 4; ELD; hELD; hOSA1; matrix-associated; MRD14; OSA1 nuclear protein; P270; SMARCF1; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily f, member 1

Database Links

Entrez Gene ID
8289
SwissProt
O14497

Additional Information

Clone
ARID1A/7735
Chromosome Location
1p36.11
Mol. Weight of Antigen
165-320kDa

Related Antibodies

View All ARID1A Antibodies

Functions

  • Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

Key References

  • Flajollet, S., et al. 2007. Mol. Cell. Endocrinol. 270: 23-32.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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