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B7-H4 (Immuno-Inhibitory Protein) Antibody [B7H4/1788]

In Stock
Catalog Number Formulation Size Price
79679-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
79679-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
79679-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescence staining of SKBR-3 cells usingB7-H4 Mouse Monoclonal Antibody (B7H4/1788); followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is Reddot.

Immunofluorescence staining of SKBR-3 cells usingB7-H4 Mouse Monoclonal Antibody (B7H4/1788); followed by goat anti-mouse IgG-CF488 (green). Nuclear counterstain is Reddot.

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with B7-H4 Mouse Monoclonal Antibody (B7H4/1788).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with B7-H4 Mouse Monoclonal Antibody (B7H4/1788).

SDS-PAGE Analysis Purified B7-H4 Mouse Monoclonal Antibody (B7H4/1788). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified B7-H4 Mouse Monoclonal Antibody (B7H4/1788). Confirmation of Purity and Integrity of Antibody.

Western Blot of HCT116 cell lysates using B7-H4 Mouse Monoclonal Antibody (B7H4/1788).

Western Blot of HCT116 cell lysates using B7-H4 Mouse Monoclonal Antibody (B7H4/1788).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane
Gene Name
VTCN1
Positive Control
HeLa, Pancreas, placenta or spleen., SKBr-3 or MCF-7 cells. Human ovary
Immunogen
A recombinant fragment of human B7-H4 protein (exact sequence is proprietary)
Alternate Names
V-set domain-containing T-cell activation inhibitor 1, B7 homolog 4, B7h.5, Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7S1; Immune costimulatory protein B7-H4; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VCTN1

Database Links

Entrez Gene ID
79679
SwissProt
Q7Z7D3

Additional Information

Clone
B7H4/1788
Chromosome Location
1p13.1-12
Mol. Weight of Antigen
35kDa

Related Antibodies

View All VTCN1 Antibodies

Functions

  • Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Key References

  • Sica, G.L., Choi, I.H., Zhu, G., Tamada, K., Wang, S.D., Tamura, H., Chapoval, A.I., Flies, D.B., Bajorath, J. and Chen, L. 2003. B7-H4, a molecule of the B7 family, negatively regulates T cell immunity. Immunity 18:849-861.
  • Salceda, S., Tang, T., Kmet, M., Munteanu, A., Ghosh, M., Macina, R., Liu, W., Pilkington, G. and Papkoff, J. 2005. The immunomodulatory protein B7-H4 is overexpressed in breast and ovarian cancers and promotes epithelial cell transformation. Exp. Cell Res. 306:128-141.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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