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BAP1 (BRCA1 Associated Protein 1) Antibody [BAP1/2433]

In Stock
Catalog Number Formulation Size Price
8314-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
8314-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
8314-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Breast carcinoma stained with BAP1 Mouse Monoclonal Antibody (BAP1/2433).

Formalin-fixed, paraffin-embedded human Breast carcinoma stained with BAP1 Mouse Monoclonal Antibody (BAP1/2433).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The BAP1 gene belongs to the ubiquitin C-terminal hydrolase subfamily of de-ubiquitination enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germ line mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, Uveal melanoma and cutaneous melanoma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Mesenchymal Stem Cell Differentiation, Nuclear Marker
Isotype / Light Chain
IgG2 / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
BAP1
Positive Control
Jurkat, HepG2 or PC-3 cells. Human testis, placenta, ovaries or breast.
Immunogen
Recombinant human BAP1 protein fragment (around aa 191-326) (exact sequence is proprietary)
Alternate Names
Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1, Cerebral protein 6, BAP1; BRCA1 associated protein 1; Cerebral protein 13; Cerebral protein 6; HUCEP 13; Hucep 6; HUCEP13; Hucep6; TPDS; Ubiquitin carboxy terminal hydrolase; Ubiquitin carboxyl terminal hydrolase BAP 1; UCHL2

Database Links

Entrez Gene ID
8314
SwissProt
Q92560

Additional Information

Clone
BAP1/2433
Chromosome Location
3p21.1
Mol. Weight of Antigen
91kDa

Related Antibodies

View All BAP1 Antibodies

Functions

  • Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922). Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) (PubMed:20436459, PubMed:25451922). Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993). Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Acts as a tumor suppressor (PubMed:9528852).

Key References

  • Thway K, et al. Am J Surg Pathol. 2012 Mar;36(3):462-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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