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BATF2 / SARI (Transcription Factor) Antibody [PCRP-BATF2-2B9]

In Stock
Catalog Number Formulation Size Price
116071-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
116071-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
116071-MSM2-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in defense response to protozoan; myeloid dendritic cell differentiation; and regulation of transcription by RNA polymerase II. Predicted to be part of chromatin. Predicted to be active in nucleus. This antibody recognizes a transcription factor involved in differentiation of CD8+ thymic dendritic cells. BATF2 has been implicated in breast cancer, malignant glioma and metastatis melanoma progression.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Nucleus.
Gene Name
Positive Control
colon or pancreas.|Human spleen
Immunogen
Recombinant fragment (around aa5-140) of human BATF2 (exact sequence is proprietary)
Alternate Names
Basic leucine zipper transcriptional factor ATF-like 2, Suppressor of AP-1 regulated by IFN, B ATF 2; Basic leucine zipper transcriptional factor ATF like 2; BATF2; MGC20410; SARI; Suppressor of AP 1 regulated by IFN

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-BATF2-2B9
Chromosome Location
11q13.1
Mol. Weight of Antigen
29.4kDa

Functions

  • AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system. Following infection, participates in the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes (By similarity). Selectively suppresses CCN1 transcription and hence blocks the downstream cell proliferation signals produced by CCN1 and inhibits CCN1-induced anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma. Possibly acts by interfering with AP-1 binding to CCN1 promoter.

Key References

  • Venkataraman A, Yang K, Irizarry J, et al. A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nat Methods. 2018;15(5):330-338. doi:10.1038/nmeth.4632

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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