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Recombinant Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Antibody [rBCL2/6418]

In Stock
Catalog Number Formulation Size Price
596-MSM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
596-MSM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
596-MSM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsilstained with Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/6418).

Formalin-fixed, paraffin-embedded human tonsilstained with Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/6418).

SDS-PAGE Analysis Purified Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/6418). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis Purified Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/6418). Confirmation of Purity and Integrity of Antibody.

BCL-2 Antibody in Western Blot (WB).

BCL-2 Antibody in Western Blot (WB).

Western Blot Analysis of HDLM-2 cell lysate using Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2/6418).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Cardiovascular, Immunology, AKT Signaling, Basal Cell Marker, Cytokine Signaling, Lung Cancer, Mitochondria Marker, Neuroinflammation, Signal Transduction
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane
Gene Name
BCL2
Positive Control
Jurkat, K562 or HeLa cells. Humantonsil or follicular lymphomas.
Immunogen
A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
Alternate Names
Apoptosis regulator Bcl-2, Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Database Links

Entrez Gene ID
596
SwissProt
P10415

Additional Information

Clone
rBCL2/6418
Chromosome Location
18q21.33
Mol. Weight of Antigen
25-26kDa

Related Antibodies

View All BCL2 Antibodies

Functions

  • Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Key References

  • Adams, J.M., et al. 1998. The Bcl-2 protein family: arbiters of cell survival. Science 281: 1322-1326.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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