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Recombinant BCL10 (MALT-Lymphoma Marker) Antibody [BL10/2988R]

In Stock
Catalog Number Formulation Size Price
8915-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
8915-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
8915-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human tonsil stained with BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R).

Formalin-fixed, paraffin-embedded human tonsil stained with BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R).

SDS-PAGE Analysis of Purified BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of PFA-fixed K562 cells. BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R) followed by goat anti-Rabbit IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed K562 cells. BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R) followed by goat anti-Rabbit IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed K562 cells labeling BCL10. BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R) followed by goat anti-rabbit IgG-CF488 (green). Phalloidin counterstain (red).

Immunofluorescence Analysis of PFA-fixed K562 cells labeling BCL10. BCL10 Recombinant Rabbit Monoclonal Antibody (BL10/2988R) followed by goat anti-rabbit IgG-CF488 (green). Phalloidin counterstain (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1;14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NF B activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1;14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Immunology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm, Membrane raft, Perinuclear region
Gene Name
BCL10
Positive Control
K562 or HepG2 cells. Human lymphoma.
Immunogen
Recombinant human BCL10 protein fragment (around aa122-168) (exact sequence is proprietary)
Alternate Names
B-cell lymphoma/leukemia 10, B-cell CLL/lymphoma 10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular homolog of vCARMEN, Cellular-E10, Mammalian CARD-containing adapter molecule E10, B-cell CLL/lymphoma 10, B-cell leukemia/lymphoma 10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, caspase-recruiting domain-containing protein, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular homolog of vCARMEN, Cellular-E10 (cE10), CIPER, hCLAP, mE10, R-RCD1

Database Links

Entrez Gene ID
8915
SwissProt
O95999

Additional Information

Clone
BL10/2988R
Chromosome Location
1p22.3
Mol. Weight of Antigen
33kDa

Related Antibodies

View All BCL10 Antibodies

Functions

  • Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation (PubMed:10187770, PubMed:10364242, PubMed:10400625, PubMed:25365219, PubMed:24074955). Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:24074955). Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex (PubMed:24074955). This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:18287044, PubMed:27777308, PubMed:24074955). Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity (PubMed:26488816). Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR) (PubMed:18264101, PubMed:18287044, PubMed:27777308, PubMed:24074955). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK (PubMed:10187815).

Key References

  • Ye H et. al. Am J Pathol 2000;157:1147-54.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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