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Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody [B2M/1118]

In Stock
Best Seller
Catalog Number Formulation Size Price
567-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
567-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
567-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118)

Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B/1118)

Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Cervical Ca stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118)

Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 4

Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118). Confirmation of Integrity and Purity of Antibody.

Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 5

Immunofluorescent staining of HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118); followed by goat anti-mouse IgG-CF488 (Green).

Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 6

Flow Cytometric Analysis of HeLa cells using Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118); followed bygoat anti-mouse IgG-CF488 (Blue); Unstained cells Control (Red).

Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 7

Western Blot Analysis of THP-1 and Raji Cell lysate using Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/1118).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Beta2 microglobulin is a 12KDa protein with a pI of 5.6. Serum beta2 microglobulin levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin’s lymphoma. In urine, increased levels are seen in proximal renal tubular disease as well as renal transplant rejection. Beta2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumours and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy).

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Non-Human primates
Research Areas
Cancer, Cardiovascular, Immuno Oncology, Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell surface, Secreted
Gene Name
B2M
Positive Control
Cervix, Endometrial, HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Kidney or Colon.
Immunogen
Full length recombinant human B2M protein
Alternate Names
Beta-2-microglobulin, B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p

Database Links

Entrez Gene ID
567
SwissProt
P61769

Additional Information

Clone
B2M/1118
Chromosome Location
15q21-q22.2
Mol. Weight of Antigen
12kDa

Related Antibodies

View All B2M Antibodies

Functions

  • Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Key References

  • Liabeuf A, le Borgne de Kaouel C, Kourilsky FM, Malissen B, Manuel Y, Sanderson AR. An antigenic determinant of human beta 2-microglobulin masked by the association with HLA heavy chains at the cell surface: analysis using monoclonal antibodies. J Immunol. 1981 Oct;127(4):1542-8.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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