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Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody [B2M/961]

In Stock
Best Seller
Catalog Number Formulation Size Price
567-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
567-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
567-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human melanoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human melanoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

SDS-PAGE Analysis Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis Purified Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of THP-1 and Raji cell lysates using Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Western Blot Analysis of THP-1 and Raji cell lysates using Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961).

Immunofluorescence Analysis of HeLa cells labeling with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (green). nuclei stained with RedDot (red).

Immunofluorescence Analysis of HeLa cells labeling with Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (green). nuclei stained with RedDot (red).

Flow Cytometric Analysis of human HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of human HeLa cells. Beta-2-Microglobulin Mouse Monoclonal Antibody (B2M/961) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Non-Human primates
Research Areas
Cardiovascular, Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell surface, Secreted
Gene Name
B2M
Positive Control
Cervix, Endometrial, HL-60 or HeLa cells. Carcinoma of Stomach, Kidney or Colon., Raji, THP-1
Immunogen
Full length recombinant human B2M protein
Alternate Names
Beta-2-microglobulin, B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p

Database Links

Entrez Gene ID
567
SwissProt
P61769

Additional Information

Clone
B2M/961
Chromosome Location
15q21-q22.2
Mol. Weight of Antigen
12kDa

Related Antibodies

View All B2M Antibodies

Functions

  • Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Key References

  • Josson, S., et al. 2011. β2-Microglobulin induces epithelial to mesenchymal transition and confers cancer lethality and bone metastasis in human cancer cells. Cancer Res. 71: 2600-2610.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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