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Printable Datasheet

Biotin (Vitamin B7 or Vitamin H) Antibody [SPM375]

In Stock
Catalog Number Formulation Size Price
MSM1X-400-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM1X-400-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM1X-400-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Biotin (Vitamin B7 or Vitamin H) Antibody

Formalin-fixed, paraffin-embedded tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin Monoclonal Antibody (SPM375).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
All species
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Biotinylated proteins in solution or on tissues.
Immunogen
Biotinylated sheep immunoglobulin
Alternate Names
N/A, N/A, Vitamin B7 or Vitamin H

Database Links

Entrez Gene ID
Not Applicable
SwissProt
N/A

Additional Information

Clone
SPM375
Mol. Weight of Antigen
244Da

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Uccellini MB, et al. 2008. J. Immunol. 181:5875.
  • Green NM, et al. 2012. J Biol Chem. 287:39789

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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