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Recombinant BRG1 / SMARCA4 Antibody [BRG1/7633R]

In Stock
Catalog Number Formulation Size Price
6597-RBM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6597-RBM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6597-RBM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human colon stained with BRG1 / Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human colon stained with BRG1 / Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human ovarian small cell carcinoma stained with BRG1 Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human ovarian small cell carcinoma stained with BRG1 Recombinant Rabbit Monoclonal Antibody (BRG1/7633R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/ SNF, including the NURF (nucleosome remodeling factor).

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology, Immunology, Cytokine Signaling, Neural Stem Cells, Nuclear Marker, Signal Transduction, Stem Cell Differentiation, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
SMARCA4
Positive Control
HeLa or HEK-293T cells. Ubiquitous nuclear expression.
Immunogen
Recombinant fragment (around aa 200-400) of human SMARCA4 protein (exact sequence is proprietary)
Alternate Names
Transcription activator BRG1, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, ATP dependent helicase SMARCA4; BAF190A; Brahma protein like 1; BRM/SWI2 related gene 1; global transcription activator snf2l4; Homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; MRD16; Nuclear protein GRB1; Protein brahma homolog 1; RTPS2; SMARCA4;; SNF2LB; SWI2; Transcription activator BRG1

Database Links

Entrez Gene ID
6597
SwissProt
P51532

Additional Information

Clone
BRG1/7633R
Chromosome Location
19p13.2
Mol. Weight of Antigen
185kDa

Related Antibodies

View All SMARCA4 Antibodies

Functions

  • Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (By similarity). In brown adipose tissue, involved in the regulation of thermogenic genes expression (By similarity).

Key References

  • Angus, S.P., et al. 2003. Retinoblastoma tumor suppressor: analyses of dynamic behavior in living cells reveal multiple modes of regulation. Mol. Cell. Biol. 23: 8172-8188.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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