Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody [BRD.3]

In Stock
Catalog Number Formulation Size Price
MSM5-885-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM5-885-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM5-885-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Mouse Monoclonal Antibody (BRD.3).

Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Mouse Monoclonal Antibody (BRD.3).

SDS-PAGE Analysis of Purified BrdU Mouse Monoclonal Antibody (BRD.3). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified BrdU Mouse Monoclonal Antibody (BRD.3). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
All species
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Immunogen
Bromodeoxyuridine (BrdU) conjugated to BSA
Alternate Names
N/A, N/A, Bromodeoxyuridine, BUdr

Database Links

Entrez Gene ID
Not Applicable
SwissProt
N/A

Additional Information

Clone
BRD.3
Mol. Weight of Antigen
Depends on the target

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
  • Williams LS et. al. Cytometry, 1990, 11(4):490-7.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales