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Recombinant c-Myc Oncoprotein Antibody [MYC/7854R]

In Stock
Catalog Number Formulation Size Price
4609-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4609-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4609-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant c-Myc Oncoprotein Antibody

Formalin-fixed, paraffin-embedded human cervix stained with c-Myc Recombinant Rabbit Monoclonal Antibody (MYC/7854R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant c-Myc Oncoprotein Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with c-Myc Recombinant Rabbit Monoclonal Antibody (MYC/7854R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant c-Myc Oncoprotein Antibody - Image 3

Formalin-fixed, paraffin-embedded human lymphoma stained with c-Myc Recombinant Rabbit Monoclonal Antibody (MYC/7854R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

c-Myc Antibody in Western Blot (WB)

c-Myc Antibody in Western Blot (WB)

Western blot analysis of K-562 lysate using c-Myc Recombinant Rabbit Monoclonal Antibody (MYC/7854R).

c-Myc Antibody in SDS-PAGE

c-Myc Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified c-Myc Recombinant Rabbit Monoclonal Antibody (MYC/7854R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a transcription factor of 64-67kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease. �

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Breast Cancer, Cardiovascular, Developmental Biology, Immunology, Bladder Cancer, Colon Cancer, Cytokine Signaling, Infectious Disease, Lung Cancer, MAPK Signaling, Nuclear Marker, Signal Transduction, Stem Cell Differentiation, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
MYC
Positive Control
HL-60 or K-562 cells. Human cervical carcinoma or heart tissue.
Immunogen
Recombinant fragment (around aa1-200) of human MYC protein (exact sequence is proprietary)
Alternate Names
Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, Class E basic helix-loop-helix protein 39 (bHLHe39), MRTL, Myc2, Niard, Nird, Proto-oncogene c-Myc, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog

Database Links

Entrez Gene ID
4609
SwissProt
P01106

Additional Information

Clone
MYC/7854R
Chromosome Location
8q24.21
Mol. Weight of Antigen
62-64kDa

Related Antibodies

View All MYC Antibodies

Functions

  • Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).

Key References

  • Evan GI, et. al. Molecular and Cellular Biology, 1985, 5(12):3610-6.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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