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Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [SPM168]

Name: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody
SKU: 800-MSM1X
Availability: InStock

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Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

Formalin-fixed, paraffin-embedded human Uterus stained with Caldesmon Monoclonal Antibody (SPM168).

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody - Image 2

Western Blot Analysis of human Ovary tissue lysate using Caldesmon MAb (SPM168).

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Western Blot (WB)

2-4ug/ml

Western Blot Protocol

Summary

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Human

Research Areas

Cardiovascular

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cytoplasm, Cytoskeleton, Myofibril, Stress fiber

Gene Name

CALD1

Positive Control

Blood vessels in all tissues, ovary, smooth muscle or leiomyosarcoma., Uterus

Immunogen

Crude human uterus extract

Alternate Names

Caldesmon, CAD; CALD1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; CDM; HCAD; LCAD; NAG22

Database Links

Entrez Gene ID

800

SwissProt

Q05682

Additional Information

Clone

SPM168

Chromosome Location

7q33

Mol. Weight of Antigen

150kDa

Functions

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

Key References

Frid MG, et al. Phenotypic changes of human smooth muscle cells during development: Late expression of heavy caldesmon and calponin. Dev Biol 1992; 153:185

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [SPM168]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

Related Antibodies

Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [rCALD1/7266]

Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [rCALD1/820]

Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [CALD1/7024R]

Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [CALD1/1424R]

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [h-CALD]

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [CALD1/820]

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [CALD1/820 + h-CALD]