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Calnexin (Endoplasmic Reticulum Marker) Antibody [CANX/1541]

In Stock
Catalog Number Formulation Size Price
821-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
821-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
821-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Endoplasmic reticulum, Endoplasmic reticulum membrane, Melanosome
Gene Name
Positive Control
HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
Immunogen
Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
Alternate Names
Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, Calnexin; CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; P90

Database Links

Entrez Gene ID
821
SwissProt

Additional Information

Clone
CANX/1541
Chromosome Location
5q35.3
Mol. Weight of Antigen
~90kDa

Functions

  • Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Key References

  • Williams, D.B. 1995. Calnexin: a molecular chaperone with a taste for carbohydrate. Biochem. Cell Biol. 73: 123-132.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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