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Recombinant Calponin-1 (Smooth Muscle Marker) Antibody [CNN1/8870R]

In Stock
Catalog Number Formulation Size Price
1264-RBM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1264-RBM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1264-RBM13-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Calponin-1 Antibody in Immunohistochemistry (IHC (P))

Calponin-1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human smooth muscle stained with Calponin Rabbit Recombinant Monoclonal Antibody (CNN1/8870R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Calponin-1 / CNN1 Antibody in SDS-PAGE

Calponin-1 / CNN1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CNN1 Recombinant Rabbit Monoclonal Antibody (CNN1/8870R). Confirmation of Purity and Integrity of Antibody.

Calponin-1 Antibody in Western Blot (WB)

Calponin-1 Antibody in Western Blot (WB)

Western Blot Analysis of Colon tissue lysate using Calponin-1 Recombinant Rabbit Monoclonal Antibody (CNN1/8870R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin.Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm.
Gene Name
CNN1
Positive Control
K562 cells. Myoepithelial cells in breast ducts, colon or leiomyosarcoma.
Immunogen
Recombinant fragment corresponding to C-terminus of human Calponin (exact sequence is proprietary)
Alternate Names
Calponin-1, Basic calponin, Calponin H1, smooth muscle, Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin-1; CNN1; Cnn1; Sm Calp; SMCC

Database Links

Entrez Gene ID
1264
SwissProt
P51911

Additional Information

Clone
CNN1/8870R
Chromosome Location
19p13.2
Mol. Weight of Antigen
33kDa

Related Antibodies

View All CNN1 Antibodies

Functions

  • Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Key References

  • Tang, D.C., et al. 1996. Structure-function relations of smooth muscle Calponin. The critical role of Serine 175. J. Biol. Chem. 271: 8605-8611.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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