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Recombinant Calretinin / Calbindin 2 (Mesothelioma Marker) Antibody [rCALB2/7123]

In Stock
Catalog Number Formulation Size Price
794-MSM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
794-MSM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
794-MSM13-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human brain stained with Calretinin Recombinant Mouse Monoclonal Antibody (rCALB2/7123). Inset: PBS instead of primary, secondary negative control.

Formalin-fixed, paraffin-embedded human brain stained with Calretinin Recombinant Mouse Monoclonal Antibody (rCALB2/7123). Inset: PBS instead of primary, secondary negative control.

Calretinin Antibody in Western Blot (WB)

Calretinin Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain tissue lysate using Calretinin Recombinant Mouse Monoclonal Antibody (rCALB2/7123).

Calretinin (Calbindin 2) Antibody in SDS-PAGE

Calretinin (Calbindin 2) Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Calretinin Recombinant Mouse Monoclonal Antibody (rCALB2/7123). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm. Nucleus.
Gene Name
CALB2
Positive Control
Human brain|testis or mesothelioma tissue.
Immunogen
Recombinant human Calretinin (Calbindin 2) protein fragment (around aa23-242) (exact sequence is proprietary)
Alternate Names
Calretinin, 29 kDa calbindin, 29kDa calbindin; CAB29; CAL2; CALB2; Calbindin 2; Calbindin D29K; Calretinin; CR

Database Links

Entrez Gene ID
794
SwissProt
P22676

Additional Information

Clone
rCALB2/7123
Chromosome Location
16q22.2
Mol. Weight of Antigen
29kDa

Related Antibodies

View All CALB2 Antibodies

Functions

  • Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Key References

  • Rogers, J.H. 1987. J. Cell Biol. 105: 1343-1353.
  • Doglioni, C., et al. 1996. Am. J. Surg. Pathol. 20: 1037-1046.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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