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Carbonic Anhydrase IX (Renal Cell Marker) Antibody [CA9/781]

In Stock
Catalog Number Formulation Size Price
768-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
768-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
768-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Carbonic Anhydrase IX (Renal Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CAIX Mouse Monoclonal Antibody (CA9/781).

Carbonic Anhydrase IX (Renal Cell Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CAIX Mouse Monoclonal Antibody (CA9/781). Confirmation of Integrity and Purity of Antibody.

Carbonic Anhydrase IX (Renal Cell Marker) Antibody - Image 3

Western Blot Analysis of HCT116 cell lysate CAIX Mouse Monoclonal Antibody (CA9/781).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Horse, Human
Research Areas
Hypoxia, Angiogenesis
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus membrane, Nucleolus, Nucleus
Gene Name
CA9
Positive Control
Normal kidney or renal cell carcinoma.
Immunogen
Recombinant human CA9 protein
Alternate Names
Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CA9, CAH9_HUMAN, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Database Links

Entrez Gene ID
768
SwissProt
Q16790

Additional Information

Clone
CA9/781
Chromosome Location
9p13.3
Mol. Weight of Antigen
55kDa

Related Antibodies

View All CA9 Antibodies

Functions

  • Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

Key References

  • Sly, W.S., et al. 1995. Annu. Rev. Biochem. 64: 375-401.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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