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Catenin, alpha-1 (CTNNA1) Antibody [1G5]

In Stock
Catalog Number Formulation Size Price
1495-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1495-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1495-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Catenin, alpha-1 (CTNNA1) Antibody

Western Blot of A431 and MCF-7 cell lysate using Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

Catenin, alpha-1 (CTNNA1) Antibody - Image 2

SDS-PAGE Analysis of Purified Catenin, alpha-1 Mouse Monoclonal Antibody (1G5).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 102kDa, identified as Catenin, alpha-1. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, -catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-catenin also regulates the beta-catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer.'

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Autophagy, Cardiovascular, Developmental Biology, BBB VCAM-1 Signaling, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Adherens junction, Cell junction, Cell membrane, Cytoplasm, Cytoskeleton
Gene Name
CTNNA1
Positive Control
A431 or MCF-7 cells., HeLa
Immunogen
Human alpha catenin fusion protein
Alternate Names
Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, Alpha E-catenin; Cadherin-associated protein; CAP102; Catenin alpha-1; CTNNA1; Renal carcinoma antigen NY-REN-13

Database Links

Entrez Gene ID
1495
SwissProt
P35221

Additional Information

Clone
1G5
Chromosome Location
5q31.2
Mol. Weight of Antigen
102kDa

Related Antibodies

View All CTNNA1 Antibodies

Functions

  • Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). May play a crucial role in cell differentiation.

Key References

  • Johnson, KR; Lewis, JE; Li, D; Wahl, J; Soler, AP; Knudsen, KA; Wheelock, MJ. P- and E-cadherin are in separate complexes in cells expressing both cadherins. Exp Cell Res 1993, 207: 252-60
  • Nieman, MT; Kim, JB; Johnson, KR; Wheelock, MJ. Mechanism of extracellular domain-deleted dominant negative cadherins. J Cell Science 1999, 112: 1621-32

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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