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Recombinant CD117 / c-Kit (Marker for Gastrointestinal Stromal Tumors) Antibody [C117/8399R]

In Stock
Catalog Number Formulation Size Price
3815-RBM21-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3815-RBM21-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3815-RBM21-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human GIST stained with CD117 Recombinant Rabbit Monoclonal Antibody (C117/8399R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human GIST stained with CD117 Recombinant Rabbit Monoclonal Antibody (C117/8399R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified CD117 Recombinant Rabbit Monoclonal Antibody (C117/8399R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified CD117 Recombinant Rabbit Monoclonal Antibody (C117/8399R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Research Areas
Breast Cancer, Cardiovascular, AKT Signaling, Cardiac Stem Cells, Dendritic Cell Marker, Hematopoietic Stem Cells, Infectious Disease, Mast Cell Marker, Mesenchymal Stem Cell Differentiation, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
KIT
Positive Control
Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.
Immunogen
Recombinant fragment (around aa 50-250) of human KIT protein (exact sequence is proprietary)
Alternate Names
Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, p145; Steel Factor Receptor; Stem Cell Factor Receptor (SCF-Receptor); Mast Cell Growth Factor Receptor

Database Links

Entrez Gene ID
3815
SwissProt
P10721

Additional Information

Clone
C117/8399R
Chromosome Location
4q12
Mol. Weight of Antigen
~145kDa

Related Antibodies

View All KIT Antibodies

Functions

  • Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Key References

  • Zafrani B, et. al. Histopathology 2000; 37(6), 536-545.
  • Harvey JM, et. al. Journal of Clinical Oncology 1999; 17(5), 1474-1481.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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