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CD11a / Integrin L / LFA-1 Chain Antibody [DF1524]

In Stock
Catalog Number Formulation Size Price
3683-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3683-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3683-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified CD11a Mouse Monoclonal Antibody (DF1524). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD11a Mouse Monoclonal Antibody (DF1524). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a protein of 180kDa, identified as CD11a (Leucocyte Workshop IV; Code 1524). CD11a complex with the 2 subunit of the integrin family, CD18, to form the cell surface heterodimer, LFA-1 or CD11a /C18 (aLbL). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. This MAb potently blocks LFA-1 dependent homotypic cell aggregation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, AKT Signaling, BBB VCAM-1 Signaling, Hematopoietic Stem Cells, Transcription Factors
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane
Gene Name
ITGAL
Positive Control
HL60, HUT-78, JY, Molt-4, U937 and human leukocytes. Human lymph nodes and tonsils.
Immunogen
Human CD11a protein
Alternate Names
Integrin alpha-L, CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide; Integrin alpha-L; ITGAL; Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain; LFA 1 alpha (LFA1A); Ly15; Ly21; p180

Database Links

Entrez Gene ID
3683
SwissProt
P20701

Additional Information

Clone
DF1524
Chromosome Location
16p11.2
Mol. Weight of Antigen
180kDa

Related Antibodies

View All ITGAL Antibodies

Functions

  • Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R (PubMed:11812992, PubMed:15528364). Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (By similarity). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).

Key References

  • Knapp et al. (Eds.), Leukocyte Typing IV, 1989: Sections N1 (pp. 543.551), N1.l (pp. 551553); N1.2 (pp. 553554); N1.3 (pp. 555558); N1.8 (pp. 566570); N1.9 (pp. 570574); N14.4 (pp. 689693).
  • Petruzzelli L et al. Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18. J Immunol 1995, 155(2):854-866.
  • Edwards CP et al. Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1. J Biol Chem 1998, 273(44):28937-44.
  • Shang XZ and Issekutz AC. Contribution of CD11a/CD18, CD11b/CD18, ICAM-1 (CD54) and -2 (CD102) to human monocyte migration through endothelium and connective tissue fibroblast barriers. Eur J Immunol 1998, 28(6):1970-1979.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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