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CD11b / MAC-1 (Microglial Marker) Antibody [ITGAM/4741]

In Stock
Catalog Number Formulation Size Price
3684-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3684-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3684-MSM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD11b / MAC-1 (Microglial Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD11b Mouse Monoclonal Antibody (ITGAM/4741). Inset: PBS instead of primary antibody; secondary only negative control.

CD11b / MAC-1 (Microglial Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CD11b Mouse Monoclonal Antibody (ITGAM/4741). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin à �M combines with the Integrin � �2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin à �M/ � �2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and �CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Neuroscience, AKT Signaling, Complement System, Cytokine Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell surface
Gene Name
ITGAM
Positive Control
Monocytes & granulocytes. Lymph nodes|spleen and tonsil (IHC).
Immunogen
Recombinant fragment (around aa900-1100) of human ITGAM protein (exact sequence is proprietary)
Alternate Names
Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; Leukocyte adhesion receptor MO1; Ly-40; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit

Database Links

Entrez Gene ID
3684
SwissProt
P11215

Additional Information

Clone
ITGAM/4741
Chromosome Location
16p11.2
Mol. Weight of Antigen
95kDa & 170kDa

Related Antibodies

View All ITGAM Antibodies

Functions

  • Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).

Key References

  • Springer T,et al.1978.Eur. J. Immunol.8:539.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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