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Recombinant CD162 (Selectin P Ligand) Antibody [PSGL1/8192R]

In Stock
Catalog Number Formulation Size Price
6404-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6404-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6404-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD162 (Selectin P Ligand) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD162 Recombinant Rabbit Monoclonal Antibody (PSGL1/8192R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant CD162 (Selectin P Ligand) Antibody - Image 2

Formalin-fixed, paraffin-embedded human spleen stained with CD162 Recombinant Rabbit Monoclonal Antibody (PSGL1/8192R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant CD162 (Selectin P Ligand) Antibody - Image 3

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using PSGL1/8192R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

CD162 Antibody in SDS-PAGE

CD162 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD162 Recombinant Rabbit Monoclonal Antibody (PSGL1/8192R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. �

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Complement System
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface.
Gene Name
SELPLG
Positive Control
Human tonsil or spleen.
Immunogen
Recombinant full-length human SELPLG (CD162) protein
Alternate Names
P-selectin glycoprotein ligand 1, Selectin P ligand, CD162; CLA; Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; PSGL1; Selectin P ligand; SELPLG

Database Links

Entrez Gene ID
6404
SwissProt
Q14242

Additional Information

Clone
PSGL1/8192R
Chromosome Location
12q24
Mol. Weight of Antigen
43-120kDa

Related Antibodies

View All SELPLG Antibodies

Functions

  • A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
  • (Microbial infection) Acts as a receptor for enterovirus 71.

Key References

  • Yang, J.et al. (1999) Thromb. Haemost.81:1.
  • Cummings, R.D. (1999) Braz. J. Med. Biol. Res.32:519.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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