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Recombinant CD22 / BL-CAM (B-Cell Marker) Antibody [rBLCAM/4108]

In Stock
Catalog Number Formulation Size Price
933-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
933-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
933-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human spleen stained with CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/4108).

Formalin-fixed, paraffin-embedded human spleen stained with CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/4108).

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rBLCAM/4108 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rBLCAM/4108 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/4108). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/4108). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, B Cell Markers, Dendritic Cell Marker, Hematopoietic Stem Cells
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane
Gene Name
CD22
Positive Control
lymph node or spleen., Raji cells or human peripheral blood lymphocytes (hPBL). Human tonsil
Immunogen
Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
Alternate Names
B-cell receptor CD22, B-lymphocyte cell adhesion molecule, Sialic acid-binding Ig-like lectin 2, T-cell surface antigen Leu-14, B-lymphocyte cell adhesion molecule (BL-CAM); B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2); SIGLEC2; T-cell surface antigen Leu-14

Database Links

Entrez Gene ID
933
SwissProt
P20273

Additional Information

Clone
rBLCAM/4108
Chromosome Location
19q13.1
Mol. Weight of Antigen
130-140kDa

Related Antibodies

View All CD22 Antibodies

Functions

  • Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Key References

  • Sato S et al. Semin Immunol 1998, 10:287-297.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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