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Recombinant CD25 / IL2RA (Activated Lymphocyte Marker) Antibody [IL2RA/4375R]

In Stock
Catalog Number Formulation Size Price
3559-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3559-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3559-RBM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD25 / IL2RA (Activated Lymphocyte Marker) Antibody

SDS-PAGE Analysis Purified CD25 Recombinant Rabbit Monoclonal Antibody (IL2RA/4375R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD25, or interleukin 2 (IL2) receptor alpha (IL2RA), and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (CD25) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolysis. CD25 (the p55 chain of the interleukin-2 receptor) is a marker of activation for a number of cell types, including T cells, B cells, and macrophages. It also appears to be a reliable immunohistochemical marker for the discrimination of neoplastic from normal/reactive mast cells, with potential as a diagnostic tool in systemic mastocytosis. CD25 is also expressed on hairy cell leukemia.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, AKT Signaling, B Cell Markers, Cytokine Signaling, Hematopoietic Stem Cells, Immune checkpoint, Mast Cell Marker, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface.
Gene Name
IL2RA
Positive Control
Hodgkin's lymphoma|Human lung carcinoma|liver|or thymus.|Tonsil
Immunogen
Recombinant fragment (around aa1-250) corresponding to human IL-2 receptor alpha
Alternate Names
Interleukin-2 receptor subunit alpha, TAC antigen, p55, IL2 Receptor alpha; IL2R alpha chain; IL2R; IL2R subunit alpha IL2RA; Interleukin-2 receptor subunit alpha; Ly43; p55; p55 chain; T Cell Growth Factor Receptor; TAC antigen; TCGFR

Database Links

Entrez Gene ID
3559
SwissProt
P01589

Additional Information

Clone
IL2RA/4375R
Chromosome Location
10p15.1
Mol. Weight of Antigen
31kDa

Related Antibodies

View All IL2RA Antibodies

Functions

  • Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Key References

  • N.A. Azab, et al. Clinical Immunology. 2008 127(2):151-157.
  • Zhang B, et al. Ann Rheum Dis 2008;67:1037-1040.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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