Printable Datasheet

CD28 Antibody [CB28]

Name: CD28 Antibody
SKU: 940-MSM1
Availability: InStock

In Stock

Flat Rate Domestic: $75 | Orders outside the US - for Order Information | Ships next business day

CD28 Antibody in Immunofluorescence (IF)

Immunofluorescent staining of PFA-fixed Jurkat cells. CD28 Mouse Monoclonal Antibody (CB28) followed by goat anti-mouse IgG-CF488 (green). Nuclei stained by RedDot (red).

CD28 Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (CB28) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

CD28 Antibody in SDS-PAGE Analysis

SDS-PAGE Analysis of Purified CD28 Mouse Monoclonal Antibody (CB28). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Summary

Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. Itis the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Functional Studies, Immunofluorescence

Species Reactivity

Human

Research Areas

Cardiovascular, Immunology, AKT Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Immune checkpoint, Infectious Disease, Signal Transduction

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cell surface, Membrane

Gene Name

CD28

Positive Control

Jurkat cells. Human lymph nodes and tonsils.

Immunogen

Recombinant human CD28 protein

Alternate Names

T-cell-specific surface glycoprotein CD28, TP44, T-cell-specific surface glycoprotein CD28; Tp44

Database Links

Entrez Gene ID

940

SwissProt

P10747

Additional Information

Clone

CB28

Chromosome Location

2q33.2

Mol. Weight of Antigen

44kDa (monomer); 88kDa (dimer)

Functions

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Key References

Scharschmidt E, et al. Mol Cell Biol. 2004;24(9):3860-73.
Jeong SH, et al. J Virol. 2004;78:6995-7003.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “CD28 Antibody”

CD28 Antibody [CB28]

CD28 Antibody in Immunofluorescence (IF)

CD28 Antibody in Flow Cytometry (FC)

CD28 Antibody in SDS-PAGE Analysis

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

Related Antibodies

CD28 Antibody [C28/75]

CD28 Antibody [C28/77]

CD28 Antibody [204.12]

CD28 Antibody [C28/1636]

CD28 Antibody [C28/74]

CD28 Antibody [C28/76]