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CD28 Antibody [204.12]

In Stock
Catalog Number Formulation Size Price
940-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
940-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
940-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Immunofluorescent staining of Jurkat cells using CD28 Mouse Monoclonal Antibody (204.12) followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to labelnuclei (red).

Immunofluorescent staining of Jurkat cells using CD28 Mouse Monoclonal Antibody (204.12) followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to labelnuclei (red).

Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (204.12) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (204.12) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

SDS-PAGE Analysis of Purified CD28 Mouse Monoclonal Antibody (204.12). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified CD28 Mouse Monoclonal Antibody (204.12). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Cow, Human, Mouse, Pig, Sheep
Research Areas
Cancer, Cardiovascular, Immuno Oncology, Immunology, AKT Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Immune checkpoint, Infectious Disease, Signal Transduction
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell surface, Membrane
Gene Name
CD28
Positive Control
Jurkat cells. Human lymph nodes and tonsils.
Immunogen
Stimulated human leucocytes
Alternate Names
T-cell-specific surface glycoprotein CD28, TP44, T-cell-specific surface glycoprotein CD28; Tp44

Database Links

Entrez Gene ID
940
SwissProt
P10747

Additional Information

Clone
204.12
Chromosome Location
2q33.2
Mol. Weight of Antigen
44kDa (monomer); 88kDa (dimer)

Related Antibodies

View All CD28 Antibodies

Functions

  • Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Key References

  • In Leucocyte typing Workshop VI. Sandilands, G. et al. Clinical and Experimental Immunology, 162(3), 516-27 (2010).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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