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CD3e, Mouse Antibody [145-2C11]

In Stock
Catalog Number Formulation Size Price
HTM1-2013-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
HTM1-2013-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
HTM1-2013-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD3e, Mouse Antibody

SDS-PAGE Analysis of Purified CD3e Hamster Monoclonal Antibody (145-2C11).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful forin vitroblocking and activation assays, as well as apoptosis induction andin vivoT cell depletion.

Product Properties & Targets

Host
Armenian Hamster
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Mouse
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
CD3E
Positive Control
MPBMC's or Thymus or Lymph Node.
Immunogen
H2Kb specific Mouse cytotoxic T lymphocyte clone BM10 37
Alternate Names
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); IMD18; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE

Database Links

Entrez Gene ID
12501
SwissProt
P22646

Additional Information

Clone
145-2C11
Chromosome Location
9 A5.2; 9 24.84 cM (Mouse)
Mol. Weight of Antigen
23kDa

Related Antibodies

View All CD3E Antibodies

Functions

  • Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.

Key References

  • Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activation)
  • Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  • Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  • Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  • Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  • Wang W, et al. 2007. J. Immunol. 178:4885. (Activation)
  • Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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