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CD3e (T-Cell Marker) Antibody [RIV9]

In Stock
Catalog Number Formulation Size Price
916-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
916-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
916-MSM6-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG3 / Kappa
Cellular Localization
Cell membrane
Gene Name
Positive Control
Jurkat cells. Tonsil and lymph node.
Immunogen
Human peripheral lymphocytes
Alternate Names
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Database Links

Entrez Gene ID
916
SwissProt

Additional Information

Clone
RIV9
Chromosome Location
11q23.3
Mol. Weight of Antigen
20kDa

Functions

  • Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

Key References

  • Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 199
  • Leerling MF et al. Quality control of anti human CD3 and CD4 monoclonal antibodies. Dev Biol Stand 1990, 71:191-200
  • Vaessen LM et al. RIV-9: a Mouse IgG3 anti-human CD3 monoclonal antibody with strong antigen modulating and T cell eliminating properties. Transplant Proc 1989, 21:1026-7
  • Ceuppens JL and Van Vaeck F. Human T cell activation induced by a monoclonal Mouse IgG3 anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI). Cell Immunol 1989, 118(1):136-46
  • Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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