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Recombinant CD44 / HCAM (Cancer Stem Cell Marker) Antibody [HCAM/2875R]

In Stock
Catalog Number Formulation Size Price
960-RBM18-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
960-RBM18-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
960-RBM18-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD44 / HCAM (Cancer Stem Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Lymph Node stained with CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R).

Recombinant CD44 / HCAM (Cancer Stem Cell Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R). Confirmation of Integrity and Purity of Antibody.

CD44 Antibody in Western Blot (WB)

CD44 Antibody in Western Blot (WB)

Western Blot Analysis of MDA-MB-231 lysate using CD44 Rabbit Recombinant Monoclonal Antibody (1HCAM/2875R)

CD44 Antibody in Western Blot (WB)

CD44 Antibody in Western Blot (WB)

Western Blot Analysis of MCF7 lysate using CD44 Rabbit Recombinant Monoclonal Antibody (HCAM/2875R)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Articular Cartilage Extracellular Matrix, Cytokine Signaling, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation, Neural Stem Cells
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus
Gene Name
CD44
Positive Control
MCF7, HeLa or MDA-MB-231 cells. Paracortex in lymph node.
Immunogen
Stimulated human leukocytes
Alternate Names
CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8); Epican; Extracellular Matrix Receptor III (ECM III); GP90 Lymphocyte Homing Adhesion Receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group; Inlu Related p80 Glycoprotein; Ly 24; MDU2; MDU3; MIC4; MUTCH I; Phagocytic Glycoprotein 1 (PGP-1)

Database Links

Entrez Gene ID
960
SwissProt
P16070

Additional Information

Clone
HCAM/2875R
Chromosome Location
11p13
Mol. Weight of Antigen
80-95kDa

Related Antibodies

View All CD44 Antibodies

Functions

  • Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).

Key References

  • Schlossman SF, et. al. Leucocyte Typing V, p1713-1719, Oxford Univ. Press, 1995.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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