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CD47 / IAP (Integrin Associated Protein) Antibody [B6H12.2]

In Stock
Catalog Number Formulation Size Price
961-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
961-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
961-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD47 / IAP (Integrin Associated Protein) Antibody

Immunofluorescent staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (B6H12.2) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Redot (red).

CD47 / IAP (Integrin Associated Protein) Antibody - Image 2

Flow Cytometric Analysis of live Jurkat cells. CD47 Mouse Monoclonal Antibody (B6H12.2) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

CD47 / IAP (Integrin Associated Protein) Antibody - Image 3

SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (B6H12.2). Confirmation of Purity and Integrity of Antibody.

CD47 / IAP (Integrin Associated Protein) Antibody - Image 4

Immunofluorescent image of live Jurkat cells. CD47 Mouse Monoclonal Antibody (B6H12.2) followed by goat anti-mouse IgG-CF488 (green).

CD47 / IAP (Integrin Associated Protein) Antibody - Image 5

Immunofluorescent image of PFA-fixed Jurkat cells.CD47 Mouse Monoclonal Antibody (B6H12.2) followed bygoat anti-mouse IgG-CF488 (green).

CD47 / IAP (Integrin Associated Protein) Antibody - Image 6

Flow Cytometric Analysis of PFA-fixed Jurkat cells.CD47 Mouse Monoclonal Antibody (B6H12.2) followed bygoat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Immune checkpoint
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
CD47
Positive Control
brain or ovarian tumors., Jurkat, MCF-7 or OVCA3 cells. Human placenta
Immunogen
Intact CD47 Purified from placenta
Alternate Names
Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen

Database Links

Entrez Gene ID
961
SwissProt
Q08722

Additional Information

Clone
B6H12.2
Chromosome Location
3q13.12
Mol. Weight of Antigen
~50kDa

Related Antibodies

View All CD47 Antibodies

Functions

  • Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Key References

  • Edris B et. al. Proc Natl Acad Sci USA 109:6656-61 (2012).
  • Willingham SB et. al. Proc Natl Acad Sci USA 109:6662-7 (2012).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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