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CD50 / ICAM3 Antibody [SPM505]

In Stock
Catalog Number Formulation Size Price
3385-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3385-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3385-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (SPM505)

Formalin-fixed, paraffin-embedded human Tonsil stained with CD50 Monoclonal Antibody (SPM505)

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Mitochondria Marker
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Membrane
Gene Name
ICAM3
Positive Control
HL-60 or THP-1 cells. Lymph node and tonsil.
Immunogen
Stimulated human leukocytes
Alternate Names
Intercellular adhesion molecule 3, CDw50, ICAM-R, ICAMR; Intercellular adhesion molecule 3 (ICAM3)

Database Links

Entrez Gene ID
3385
SwissProt
P32942

Additional Information

Clone
SPM505
Chromosome Location
19p13.2
Mol. Weight of Antigen
110-160kDa

Related Antibodies

View All ICAM3 Antibodies

Functions

  • ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).

Key References

  • Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1546-1547, 1578-1579.
  • C.L. Holness, et al, (1995) J Biol Chem 270: 877-884.
  • Y. van Kooyk, et al, (1996) J Exp Med 183: 1247-1252.
  • Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 403-409.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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