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CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [123C3.D5 + 123A8]

Name: CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody
SKU: 4684-MSM3
Availability: InStock

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CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5 + 123A8)

CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD56 Monoclonal Antibody (123C3.D5 + 123A8)

CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody - Image 3

Flow cytometry of lymphocyte gated PBMCs unstained (gray) or stained with CF568-labeled CD56 monoclonal antibody (123C3.D5 + 123A8) (orange).

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Species Reactivity

Human

Research Areas

Autophagy, Cardiovascular, Immunology, Neuroscience, Signal Transduction

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cell membrane, Secreted

Gene Name

NCAM1

Positive Control

Cerebellum, Neuroblastoma., Pancreas, PBMCs

Immunogen

Membrane preparation of a small cell lung carcinoma

Alternate Names

Neural cell adhesion molecule 1, NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule

Database Links

Entrez Gene ID

4684; 24586

SwissProt

P13591; P13592

Additional Information

Clone

123C3.D5 + 123A8

Chromosome Location

11q23.1

Mol. Weight of Antigen

145 and 125kDa|180

Functions

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
(Microbial infection) Acts as a receptor for rabies virus.
(Microbial infection) Acts as a receptor for Zika virus.

Key References

Gerardy-Schahn R; et. al. Int Journal of Cancer. Supplement, 1994, 8:38-42.
Michalides R; et. al. International Journal of Cancer. Supplement, 1994, 8:34-7.
Schol DJ; et. al. Int J Cancer. Supplement, 1988, 2:34-40

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [123C3.D5 + 123A8]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

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Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [NCAM1/2217R]

Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [NCAM1/8392R]

Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [rNCAM1/8655]

Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [NCAM1/9121R]

CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [NCAM/7522]