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Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody [NCAM1/2217R]

In Stock
Catalog Number Formulation Size Price
4684-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4684-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4684-RBM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R).

Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R). Confirmation of Integrity and Purity of Antibody.

Recombinant CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) Antibody - Image 3

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD56 monoclonal antibody (NCAM1/2217R) (green).

CD56 (Neuronal Cell Marker) Antibody in Western Blot (WB)

CD56 (Neuronal Cell Marker) Antibody in Western Blot (WB)

Western blot analysis of Human Brain tissue lysates using CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Autophagy, Cardiovascular, Developmental Biology, Immunology, Neuroscience, Cytokine Signaling, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation, Neural Stem Cells, Signal Transduction, Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
NCAM1
Positive Control
Human Colon or Small Intestine, Brain PBMCs.
Immunogen
Recombinant full-length human NCAM1 (CD56) protein
Alternate Names
Neural cell adhesion molecule 1, NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule

Database Links

Entrez Gene ID
4684
SwissProt
P13591; P13592

Additional Information

Clone
NCAM1/2217R
Chromosome Location
11q23.1
Mol. Weight of Antigen
145 and 125kDa|180

Related Antibodies

View All NCAM1 Antibodies

Functions

  • This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • (Microbial infection) Acts as a receptor for rabies virus.
  • (Microbial infection) Acts as a receptor for Zika virus.

Key References

  • Hilkens et al., Int J Cancer. 1984 34:197-206.
  • Kibbelaar et al. Eur. J. Cancer 1991 27:431-435.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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