CD57 / B3GAT1 (Natural Killer Cell Marker) Antibody [NK1/7565]

Name: CD57 / B3GAT1 (Natural Killer Cell Marker) Antibody
SKU: 27087-MSM5
Availability: InStock

In Stock

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SDS-PAGE Analysis of Purified CD57 Mouse Monoclonal Antibody (NK1/7565). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human stomach stained with CD57 Mouse Monoclonal Antibody (NK1/7565). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Immunohistochemistry

Species Reactivity

Human

Research Areas

B Cell Markers

Isotype / Light Chain

IgG2 / Lambda

Cellular Localization

Cell surface

Gene Name

B3GAT1

Positive Control

Human lymph node or tonsil.

Immunogen

Recombinant fragment (around aa1-200) of human B3GAT1 / CD57 protein (exact sequence is proprietary)

Alternate Names

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1, 3-glucuronyltransferase 1, Glucuronosyltransferase P, UDP-GlcUA:glycoprotein beta-1, 3-glucuronyltransferase, 3-Glucuronyltransferase 1; B3GAT1; Galactosylgalactosylxylosylprotein 3-beta-Glucuronosyltransferase 1; GLCATP; GlcUAT-P; Glucuronosyltransferase P; UDP GlcUA Glycoprotein beta 1, 3 Glucuronyltransferase

Database Links

Entrez Gene ID

27087

SwissProt

Q9P2W7

Additional Information

Clone

NK1/7565

Chromosome Location

11q25

Mol. Weight of Antigen

~110kDa

Functions

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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