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CD64 Antibody [10.1]

In Stock
Catalog Number Formulation Size Price
2209-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2209-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2209-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow cytometry of U937 cells stained with CD64 monoclonal antibody (10.1) (red) or isotype control (gray) followed by goat anti-mouse CF640R (red).

Flow cytometry of U937 cells stained with CD64 monoclonal antibody (10.1) (red) or isotype control (gray) followed by goat anti-mouse CF640R (red).

Flow cytometry of monocyte gated PBMCs stained with CD64 monoclonal antibody (10.1) (red) or isotype control (gray) followed by goat anti-mouse CF640R (red).

Flow cytometry of monocyte gated PBMCs stained with CD64 monoclonal antibody (10.1) (red) or isotype control (gray) followed by goat anti-mouse CF640R (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Three different classes of IgG Fc receptors have been described: Fc stimulation. CD64 plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64 has been shown to associate with signal transducing subunit of the high affinity IgE receptor. Src family kinases Hck and Lyn show increased kinase activity and will co-immunoprecipitate with CD64 subsequent to receptor cross linking.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
FCGR1A
Positive Control
U937 cells. Human tonsil.
Immunogen
FCG10.1A / CD64 antibody was raised against rheumatoid synovial fluid cells and fibronectin Purified human monocytes.
Alternate Names
High affinity immunoglobulin gamma Fc receptor I, Fc-gamma RI, Fc-gamma RIA, CD64; CD64A; CD64b; CD64c; Fc fragment of IgG, high affinity Ia, receptor (CD64); FCGR1; FCGR1A; FCGR1B; FCGR1C; FcRI; FcRIB; FCRIC; High affinity immunoglobulin gamma Fc receptor IB; IGFR1; IGFRB; IGFRC

Database Links

Entrez Gene ID
2209
SwissProt
P12314

Additional Information

Clone
10.1
Chromosome Location
1q21.2
Mol. Weight of Antigen
43kDa

Related Antibodies

View All FCGR1A Antibodies

Functions

  • High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.

Key References

  • Townsend, S.M., et al. 2003. Infect. Immun. 71: 5871-5880.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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