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CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Antibody [TFRC/1839]

In Stock
Catalog Number Formulation Size Price
7037-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7037-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7037-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
How do I understand the HuProtTM Validation Results? Learn More
Formalin-fixed, paraffin-embedded human Placenta stained with CD71 Mouse Monoclonal Antibody (TFRC/1839).

Formalin-fixed, paraffin-embedded human Placenta stained with CD71 Mouse Monoclonal Antibody (TFRC/1839).

SDS-PAGE Analysis of Purified CD71 Mouse Monoclonal Antibody (TFRC/1839). Confirmation of Integrity and Purity of Antibody

SDS-PAGE Analysis of Purified CD71 Mouse Monoclonal Antibody (TFRC/1839). Confirmation of Integrity and Purity of Antibody

Analysis of Protein Array containing >19,000 full-length human proteins using CD71 Mouse Monoclonal Antibody (TFRC/1839) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Analysis of Protein Array containing >19,000 full-length human proteins using CD71 Mouse Monoclonal Antibody (TFRC/1839) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cancer, Cardiovascular, Signal Transduction
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Melanosome, Secreted
Gene Name
TFRC
Positive Control
HeLa, Jurkat, MCF-7 or K562 cells. Human placenta or Bone Marrow.
Immunogen
Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)
Alternate Names
Transferrin receptor protein 1, T9, p90, Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Database Links

Entrez Gene ID
7037
SwissProt
P02786

Additional Information

Clone
TFRC/1839
Chromosome Location
3q29
Mol. Weight of Antigen
85-95kDa (monomer); 190kDa (dimer)

Related Antibodies

View All TFRC Antibodies

Functions

  • Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).
  • (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Key References

  • Oudermans et al. Cancer, 1986; 58:1252.
  • K. Moolenaar et al. Cancer research 50,1102-1106, 1990.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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