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Recombinant CD71 / Transferrin Receptor (TFRC) (Extracellular Domain) Antibody [TFRC/2898R]

In Stock
Catalog Number Formulation Size Price
7037-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7037-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7037-RBM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded Human Bone Marrow stained with CD71 Rabbit Recombinant Monoclonal Antibody (TFRC/2898R).

Formalin-fixed, paraffin-embedded Human Bone Marrow stained with CD71 Rabbit Recombinant Monoclonal Antibody (TFRC/2898R).

SDS-PAGE Analysis of Purified CD71 Rabbit Recombinant Monoclonal Antibody (TFRC/2898R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified CD71 Rabbit Recombinant Monoclonal Antibody (TFRC/2898R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Melanosome, Secreted
Gene Name
TFRC
Positive Control
HeLa, Jurkat, MCF-7 or K562 cells. Human placenta or Bone Marrow.
Immunogen
Recombinant human extracellular TFRC protein fragment (aa94-212) (exact sequence is proprietary)
Alternate Names
Transferrin receptor protein 1, T9, p90, Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR

Database Links

Entrez Gene ID
7037
SwissProt
P02786

Additional Information

Clone
TFRC/2898R
Chromosome Location
3q29
Mol. Weight of Antigen
85-95kDa (monomer); 190kDa (dimer)

Related Antibodies

View All TFRC Antibodies

Functions

  • Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).
  • (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.

Key References

  • K. Moolenaar et al. Cancer research 50,1102-1106, 1990
  • Oudermans et al. Cancer, 1986; 58:1252

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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